Fig. 3. Effects of one- and 12-week exposures of BEAS-2B cells to TPM from 3R4F reference cigarette smoke (7.5 µg/mL) and THS 2.2 aerosol (7.5 µg/mL, 37.5 µg/mL, and 150 µg/mL) on (A) mitochondrial membrane potential (ΔΨm) measured using a JC-1 molecular probe and (B) cytosolic calcium levels measured using a Fluo-4 molecular probe. Values represent the means ± SD from at least three independent experiments. *p<0.05, **p<0.01, ***p<0.001 compared with vehicle (DMSO)-treated cells. NT, non-treated (control) cells.